The role of xylulose 5-phosphate in xylose metabolism of Lactobacillus pentosus.

Complementation of the inability of Lactobacillus strains to utilize D-xylose with D-xylose catabolism-encoding genes of Lactobacillus pentosus.

The inability of two Lactobacillus strains to ferment D-xylose was complemented by the introduction of Lactobacillus pentosus genes encoding D-xylose isomerase, D-xylulose kinase, and a D-xylose catabolism regulatory protein. This result opens the possibility of using D-xylose fermentation as a food-grade selection marker for Lactobacillus spp.

Conversion of D-xylose to D-xylulose in extracts of Lactobacillus pentosus.

Movement of Tritiated Water in the Root System of Helianthus annuus in the Presence and Absence of Transpiration.

22. SIMPSON, F. J., M. J. WOLIN, AND W. A. WOOD. 1958. Degradation of L-arabanose by Aerobacter aerogenes. I. A pathway involving phosphorylated intermediates. J. Biol. Chem. 230: 457-72. 23. SMITH, I. 1958. Amino acids, amines and related compounds. In: Chromatographic Techniques. I. Smith, ed. William Heinemann Medical Books, Ltd., London. p 169. 24. STARLINGKR, P. 1963. The formation of gala...

Enzymatic formation of xylulose 5-phosphate from ribose 5-phosphate in spleen.

olizing this substrate in the presence of ATP1 to form a mixture of n-ribose 5-phosphate and n-ribulose 5-phosphate. Although neither n-xylose 5-phosphate nor n-xylulose 5-phosphate was found, these authors postulated the latter as an intermediate upon the observation that n-xylulose was phosphorylated more rapidly than n-xylose. n-Xylulose 5-phosphate was first isolated and identified as a pro...

Expression of the xylulose 5-phosphate phosphoketolase gene, xpkA, from Lactobacillus pentosus MD363 is induced by sugars that are fermented via the phosphoketolase pathway and is repressed by glucose mediated by CcpA and the mannose phosphoenolpyruvate phosphotransferase system.

Purification of xylulose 5-phosphate phosphoketolase (XpkA), the central enzyme of the phosphoketolase pathway (PKP) in lactic acid bacteria, and cloning and sequence analysis of the encoding gene, xpkA, from Lactobacillus pentosus MD363 are described. xpkA encodes a 788-amino-acid protein with a calculated mass of 88,705 Da. Expression of xpkA in Escherichia coli led to an increase in XpkA act...